|
Class Lecture Notes and Laboratory Exercises
Class Notes For Analytical Biochemistry (CHM464)
Class Notes For MCB598
Undergraduate NMR Lab Exercises
Protein Titration | Sequence-specific Assignment of Proteins
NMR Titration of Functional Groups in Proteins
Literature Reference: "NMR Titration Used to Observe Specific Proton Dissociation in Polyprotic Tripeptides,"
J.L. Yarger, R.A. Nieman and A.L. Bieber, J. Chem. Ed., 74, 243-246 (1997).
The paper describes how NMR can be used to simultaneously observe the protonation state of different ionizing functional groups in peptides and proteins. You can download the NMR spectra used in this study by clicking on the links below. The spectra were obtained at 400 MHz using a Varian UnityPlus spectrometer equipped with a 5mm pulsed-field gradient indirect-detection probe.
Spectra are best processed on Sun or Silicon Graphics workstations using VNMR,
Spectra can be processed on PCs with:
MestRe-C (free, recommended),
PCNMR forWindows,
Nuts.
Spinworks
Spectra can be processed on Macs using several programs described in Peter Lundberg's list.
Spectra for processing on unix workstations have been saved as tarfiles. Download the .tar file to your workstation, then type tar-xvf filename.tar. Spectra for processing on PCs or Macs have also beensaved as compressed .zip files. For PCs, download the spectra and unzip them using somethinglike winzip.exe, available from many download sites (e.g., www.tucows.com). You can use winzip.exe to download and extract the unix tar files on a pc. The standard compression utility for Macintosh, Stuffit Expander, will also handle both the compressed .zip and uncompressed .tar files.
If you have trouble downloading these files, contact Brian.R.Cherry@asu.edu.
UNIX TAR FILES:
ZIP FILES
|
