ASU - CHM 343 - 1 semester Physical Chemistry Laboratory
Experiment #1 -
Determining the Diffusion in Liquids Using NMR: An Introduction to Data & Error Analysis
Prof. J.L. Yarger, Spring 2013.
Lab Handout (Prof. Yarger)
NMR Data (1H PFG-SE NMR data of a mixture of solutes in deuterated solvents)
Example Data (1H PFG-SE NMR data of CHCl3 in d6-Acetone)
Student Data (1H PFG-SE NMR data of a mixture of solutes in CDCl3, read me file with parameters and sample info, data in ascii format)

ASU - CHM 343 - 1 semester Physical Chemistry Laboratory
Experiment #3 -
Keto-Enol Equilibrium Using NMR

Using proton NMR spectroscopy and computational analysis to evaluate the equilibrium composition of various keto-enol mixtures. Determine the effect of solvent and temperature on the keto-enol equilibria of one or more 1,3 dicarbonyl compounds. Use this information to determine the thermodynamic change (i.e., DG, DH and DS) for the keto-enol reaction and discuss the effect of solvent on the keto-enol equilibrium.

Prof. J.L. Yarger, Spring 2013.
Lab Handout (Prof. Yarger)
Student Data (1H NMR data of AcAc in various deuterated solvents, read me file with parameters and sample info, data in ascii format)

Varian NMR Projects/Tutorials - CHM 598 - "Method in Magnetic Resonance"
Prof. J.L. Yarger, Fall 2007.

NMR of Gramicidin-S (ASU CHM 464) - Biophysical Chemistry

Class Notes:
Lecture Notes Week 1
Lecture Notes Week 2

NMR Titration of Functional Groups in Proteins
Literature Reference:  "NMR Titration Used to Observe Specific Proton Dissociation in Polyprotic Tripeptides," J.L. Yarger, R.A. Nieman and A.L. Bieber, J. Chem. Ed., 74, 243-246 (1997).
The paper describes how NMR can be used to simultaneously observe the protonation state of different ionizing functional groups in peptides and proteins.  You can download the NMR spectra used in this study by clicking on the links below.  The spectra were obtained at 400 MHz using a Varian UnityPlus spectrometer equipped with a 5mm pulsed-field gradient indirect-detection probe.
ZIP FILES

  • 2D gcosy and noesy of glytyrgly, glutyrglu, lystyrlys.  12.5 MBytes    2D.zip
  • 1D of TSP (reference).  0.07 Mb     TSP1d.zip
  • 1D of glutyrglu vs pH.   0.8 Mb     glutyrglu1d.zip
  • 1D of glytyrgly vs pH.  0.9 Mb     glytyrgly1d.zip
  • hpgl plots of spectra (can be imported in MSWord, CorelDraw, Photoshop,etc.).  0.3 Mb     hpgl_plots.zip
  • 1D of lystyrlys vs pH.   1.0 Mb     lystyrlys1d.zip
  • T1 of lystyrlys at several pH values.  2.4 Mb    t1_studies.zip
  • 1D of tyr vs pH.    0.6 Mb     tyr.zip

NMR of Gramicidin-S (ASU CHM 464) - Biophysical Chemistry

Class Notes:
Lecture Notes Week 1
Lecture Notes Week 2